Analysis Note

Appearance (clearness): clearAppearance (color): greenpH-value (25 °C): 7.0 - 7.4Growth promotion test in accordance with the current version of DIN EN ISO 11133.Mixed inoculum E.coli resp. Salmonella and E.faecalis.Additionaly monoculture of E.faecalisConfirmation on VRBD agar resp. Tryptic soy agarInoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≤ 100Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≤ 100Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): ≤ 100Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 1000Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): ≥ 1000Growth on VRBD-Agar(Escherichia coli ATCC 8739 (WDCM 00012)): >10 coloniesGrowth on VRBD-Agar(Escherichia coli ATCC 25922 (WDCM 00013)): >10 coloniesGrowth on VRBD-Agar(Salmonella typhimurium ATCC 14028 (WDCM 00031)): >10 coloniesGrowth on VRBD-Agar(Salmonella enteritidis ATCC 13076 (WDCM 00030)): >10 coloniesGrowth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total inhibitionGrowth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)): total inhibitionColonies (Escherichia coli ATCC 8739 (WDCM 00012)): pink to red colonies with or without precipitation haloColonies (Escherichia coli ATCC 25922 (WDCM 00013)): pink to red colonies with or without precipitation haloColonies (Salmonella typhimurium ATCC 14028 (WDCM 00031)): pink to red colonies with or without precipitation haloColonies (Salmonella enteritidis ATCC 13076 (WDCM 00030)): pink to red colonies with or without precipitation haloIncubation:EE broth: 24 ± 2 hours at 37 ± 1 °C aerobicConfirmation on VRBD agar resp. Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 °C aerobic

Application

Granucult^® Enterobacteriaceae Enrichment Mossel broth is intended for the selective enrichment of Enterobacteriaceae from food and animal feed and other materials.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

General description

Granucult^® Enterobacteriaceae Enrichment Mossel broth is intended for the selective enrichment of Enterobacteriaceae. The undesired accompanying bacterial flora is inhibited by brilliant green and ox bile. Glucose favors the growth of all Enterobacteriaceae, and the high buffering capacity of the culture medium prevents the formed acid from killing the culture. Glucose is the main energy source and supports the rapid growth of all Enterobacteriaceae, while enzymatic digest of animal tissue provides nitrogen, vitamins, and amino acids.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

5 kg in Plastic drum

500 g in Plastic bottle